Composite

Part:BBa_K2992030:Design

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-25)


Acetone pathway: csp_Pfdx-5-UTR+RBS-ca_thl-cb_ctfAB-cp_TFdx


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 569
    Illegal PstI site found at 3464
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3464
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 569
    Illegal PstI site found at 3464
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 569
    Illegal PstI site found at 3464
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplified unmodified from the genome sequences of the host organisms. The Nottingham 2018 entry for Pfdx possesses a c14t SNP which has been corrected for our 2019 entry.


Source

C. acetobutylicum, C. botulinum, C. pasteurianum and C. sporogenes genomic DNA.

References